How to Collect a Urine Sample from your Dog

Urine samples need to be as clean as possible (no dirt, mulch, grass, etc.), and we’ll need to examine the sample within 24 hours of collection. If you can’t bring the sample right over to the hospital, keep it refrigerated until you can bring it in.

Major tips for collecting a urine sample from your dog:

Any container you use needs to be completely clean and totally dry. A sterile urine cup is best (stop by and pick one up). You can also use a plastic soft butter tub or tight-sealing refrigerator dish (wash very well with hot soapy water, rinse well and dry thoroughly, or put it through your dishwasher before using).

Since some dog urine can contain Leptospirosis (a contagious disease that humans can get), we recommend always handling dog urine samples with gloves. This includes cleaning up accidents at home!

We really only need one tablespoon – enough to cover the bottom of a urine cup, but we will accept whatever you can get us (we may not be able to run a complete urinalysis on a too-small sample, but we’ll get what we can).

We need a liquid sample. Off the floor will not usually be able to be analyzed, but if this is the only sample you can get, try soaking the puddle of urine onto ½ of a clean single piece of paper towel. It should be dripping wet; if this is a small puddle, use ¼ of a piece of paper towel. Put the dripping paper towel into a clean ziplock bag and bring it in. We may be able to get enough urine to analyze with this method.

Miscellaneous helpful things to know about collecting a urine sample from your dog:

Always have your dog on leash, so you have control. If possible, have one person hold the leash while another collects the sample. It may take a few tries! The person who is going to collect the sample should wear gloves.

For male dogs:

  • Remove the cover of the container and keep it clean and handy (don’t set it down anywhere, and don’t put it in your pocket). The container and lid need to be kept as clean as possible.
  • Follow the dog closely, and when he begins to urinate, slip the cup under him from the side of the dog.
  • Put the cover on the container immediately and screw it tight so nothing is spilled.

For female dogs:

  • Remove the cover of the container and keep it handy (don’t set it down anywhere, and don’t put it in your pocket). The container and lid need to be kept as clean as possible.
  • Follow the dog closely, and when she squats, slip the cup under her from behind.
  • For short or small dogs, use the urine cup lid (it will hold enough).
  • Put the cover on the container immediately and screw it tight so nothing is spilled.

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